human egf enzyme-linked immunosorbent assay (elisa) kit Search Results


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Mabtech Inc ifn-α pan enzyme-linked immunosorbent assay kit
EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked <t>immunosorbent</t> assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.
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A&G Pharmaceutical human progranulin sandwich enzyme-linked immunosorbent assay (elisa) kit
EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked <t>immunosorbent</t> assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.
Human Progranulin Sandwich Enzyme Linked Immunosorbent Assay (Elisa) Kit, supplied by A&G Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abnova human enzyme-linked immunosorbent assay [elisa] kit
EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked <t>immunosorbent</t> assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.
Human Enzyme Linked Immunosorbent Assay [Elisa] Kit, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked immunosorbent assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.

Journal: Blood Advances

Article Title: Plasmacytoid dendritic cells respond to Epstein-Barr virus infection with a distinct type I interferon subtype profile

doi: 10.1182/bloodadvances.2018025536

Figure Lengend Snippet: EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked immunosorbent assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.

Article Snippet: IFN-α Pan and IL-12p40 enzyme-linked immunosorbent assay kits were purchased from Mabtech AB, and assays were performed according to the manufacturer’s recommendations.

Techniques: Infection, Enzyme-linked Immunosorbent Assay

EBV infection elicits type I IFN production in huNSG mice. huNSG mice were infected with 5 × 105 RGU EBV, and blood samples were analyzed 18 hours postinfection. IFN-α (A) or IL-12p40 (B) production was measured in the serum by enzyme-linked immunosorbent assay (PBS, n = 11; EBV, n = 12). (C) IFN-α levels were compared in the serum of huNSG mice that were PBS treated (n = 6) or infected with either 5 × 105 RGU of wild-type EBV (wt, n = 6), EBNA3A-deficient EBV (3aKO, n = 6), or EBNA3C-deficient EBV (3cKO, n = 8). (D) ISG induction was measured in blood leukocyte lysates by using quantitative real-time polymerase chain reaction. n = 8 for PBS; n = 9 for EBV. Unpaired Student t tests were applied.

Journal: Blood Advances

Article Title: Plasmacytoid dendritic cells respond to Epstein-Barr virus infection with a distinct type I interferon subtype profile

doi: 10.1182/bloodadvances.2018025536

Figure Lengend Snippet: EBV infection elicits type I IFN production in huNSG mice. huNSG mice were infected with 5 × 105 RGU EBV, and blood samples were analyzed 18 hours postinfection. IFN-α (A) or IL-12p40 (B) production was measured in the serum by enzyme-linked immunosorbent assay (PBS, n = 11; EBV, n = 12). (C) IFN-α levels were compared in the serum of huNSG mice that were PBS treated (n = 6) or infected with either 5 × 105 RGU of wild-type EBV (wt, n = 6), EBNA3A-deficient EBV (3aKO, n = 6), or EBNA3C-deficient EBV (3cKO, n = 8). (D) ISG induction was measured in blood leukocyte lysates by using quantitative real-time polymerase chain reaction. n = 8 for PBS; n = 9 for EBV. Unpaired Student t tests were applied.

Article Snippet: IFN-α Pan and IL-12p40 enzyme-linked immunosorbent assay kits were purchased from Mabtech AB, and assays were performed according to the manufacturer’s recommendations.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction